We also introduce a new section of the guide, “Cryo-EM Foundations”. In this section, we will add discussions around cryo-EM theory which are foundational for understanding how the physical and computational processes behind imaging and reconstruction work. The first entries in this section provide an introduction to the physics and mathematics underlying contrast in cryo-EM, an introduction to thinking about waves as vectors, and a discussion of the broad topic of aliasing. We hope these pages serve as a useful reference and starting point for CTF and other topics in the future.
As always, please feel free to ask questions about these or any other topic here on the discussion forum. The questions we receive and conversations we read here guide us toward topics which need more explanation and background — the forum is just as useful for us as we hope it is for you!
Do we expect that astigmatism will vary between patches? I would have expected just refining defocus might be better, leaving astigmatism constant across the micrograph - is there evidence that refining astigmatism patch-wise helps?
@team, you are doing so great things for the community! I praise your commitment to educate and help us all!
The work, time and effort you have been doing here since the very beginning of this discussion forum and with the CryoSPARC guide is amazing!
Thank you very much and congratulations on another milestone!
Working on an interesting case example for this at the minute as it’s something that cropped up when working on a different idea. Not sure it will get published, but I hope so.
Hi @olibclarke! Although @rbs_sci’s data sounds interesting, in this case this was an error on my part. The defocus is refined per-patch, while the astigmatism is refined per-micrograph. Thanks for catching that! I’ve corrected it now.
