Hi @yasharsadian, it looks like one of particle dataset files has invalid data in it. Did you do any processing of these particles outside of cryoSPARC? Can you direct-message me the particle dataset files that were used as an input for the 2D Classification job? You can download them from the “Outputs” group of the parent job (e.g., the Extract from Micrographs job):
Please send me both the download results of the particle.blob
and particle.location
outputs.