Unable to Replicate Homogeneous Refinement Results in 3D Auto Refine in Relion

Hi everyone,

I’ve been trying to replicate the results of a homogeneous refinement job result from cryosparc in relion’s 3D auto refine and I’ve been unable to achieve the same resolution. I’ve attempted different masks including the same one outputted from cryosparc and relion made masks and I’ve also tried local angular searches as well. Does anyone have any input on how to best replicate the results from cryosparc in relion 3D autorefine?


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