Tutorial: Yeast U4/U6.U5 tri-snRNP Initial processing problem

Cryo-EM Data Processing
1

Hello,

I am new to CryoSPARC and so am working through your data processing tutorials. In the beginning of this Yeast U4/U6.U5 tri-snRNP tutorial it says to run Ab-Initio and Homogenous Refinement with default parameters and you should get about 3.7 angstrom resolution. For some reason I am getting about 6 angstrom resolution.

I am using CryoSPARC v4.0.2.

For import I got the .star file from EMPIAR with the accompanying .mrcs, and imported those using hte import particles job with parameters from the paper. (Accerating voltage 300kV, spherical aberration 2.7mm, pixel size 1.43 Angstroms)

I am wondering if this is due to the version update (less overfitting) or if I have a parameter or step wrong?

Thank you