Hi CS users,
I am working with a small protein and it exists as a dimer in solution (128kDa), I collected krios data and using its homolog structure, measured the size in chimera, the maximum size is 11 nm across the diagonal. I am new to cryo-EM and i am finding it difficult to locate the protein in the micrograph. And the 2D using blobpicker gave only the noisy fuzzy 2D. Any recommendations are highly welcome. Thanks in advance.
What magnification was that micrograph taken at? That will at least give us a rough idea of how large your target might be on the micrograph…
Try denoising the micrographs (train the model, don’t use the pre-trained one! Increasing training iterations might help also) and see if you can see anything more convincing on the micrographs, because there is nothing on that micrograph which looks promising to me.
130kX , 0.68 A/pixel
At that mag there is nothing there that looks right. Let’s see what the denoised mics look like. 
