I’m working with a membrane protein that shows some flexibility induced loss of feature in some regions.
Because of that I was thinking to collect a few tilt-pair movies, to get more thorough understanding about the features of the particles.
However, I was wondering how to treat a tilt-pair data in cryosparc. Unlike EMAN2, cryosparc does not have any tilt pair boxer program.
Some suggestions on this matter would be very helpful.