Hi all,
I’m working on a filement-like protein structure. The protein seem like a filament, and by using the filament tracer I can successfully pick particles from the micrographs.
The question is the protein feature from 2D classification seem very heterogeneity, many filament seem curved，even I use small box size (192) extract particles, the problem still exist.
I would like to get more straight filament from the 2D classification，any suggestion for improvment？
