Should I use symmetry T (Tetrahedral) for all tetrahedral structures? Like for the structure in PDB entry 8TL7, it has a T3 symmetry, should a symmetry of T3 be applied? Seems cryosparc does not provide this option. I’m not an expert in this so any information would be appreciated
Hello Ghdel,
Have you tried just putting T as symmetry operator? This is what they mention in the methods of that paper.
I haven’t had much experience with symmetric proteins. I noticed that in some cases they obtain nice maps with the symmetry imposed, and then relax (use C1) to improve the resolution in specific parts. In some other cases it doesn’t help, so you keep imposing symmetry until the end.
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