Dear cryosparc community,

Would you please explain how the local refinement works after symmetry expansion in cryosparc? I see that the number of the particles is multiplied according to symmetry group with symmetry expand job . Will, then, local refinement use all the multiplied particles to align locally? or will local refinement choose a single (best fitting) particle from the each symmetry multiplied particle? I need some enlightment for this process.

Another question. I transferred particles from relion with refinement angles and tried to do local refinement but it did not work, it gave a non optimal input error. I saw couple of posts here claiming that imported particles with angles can now be used directly to run a local refinement in cryosparc. Would you know what would be going wrong? I have 2.15.0.

I have a pseudosymmetric particle and refinement mixes up monomers. I can separate the particle sets with 3d classification without alignment in relion but I need to be able to bring back those particles to cryosparc and do local refinement directly. I would appreciate your input on this one.

Thank you.