From cryosparc v 2.9, it is possible to import the symmetry expanded particles from relion.star files and proceed to the local refinement job. This is a very useful function.
recently I was trying to import the expanded particles and conduct particle subtraction in cryosparc, however, it seems the particle subtraction job didn’t read the orientation of imported particles correctly. For example, when the imported particles are expanded from C2 symmetry (within relion), the output of subtraction job (mask of the subtraction is non-symmetic) always shows that two particles are identical, while the same particles.star can lead to correct output directly from local refinement job.
I am wondering if there is a way to do the subtraction job with imported symmetry-expanded particles correctly?