Symmetry-Driven Strategies: Tips & Tricks for Resolving Ligand Density in C1 Symmetry Along the C4 Axis

Correct, supplied orientations are not used in NU or homo refinement. Currently there is no way to apply symmetry relaxation in local refinement in CS, only in relion (I have requested this for CS: Symmetry relaxation in v4.4 - local refinement implementation?).