Super-resolution pixel size resulting in small map

Cryo-EM Data Processing
1

Hello,

I am working with a dataset collected on a Krios with a K2, and a pixel size of 1.114. Using super-resolution, the pixel size is 0.557.

Importing my movies with the super-resolution pixel size, I’m getting a product map that is half the size of the map I get from the regular pixel size. Related structures make it clear that the regular pixel size map is the correct one, and the super-resolution map is too small.

Am I implementing super-resolution wrong in some way or am I skipping a step? In principle, I understand why this is happening, but can’t sort out a way around it. I also assume this is wildly throwing off my resolution estimates, as I am getting GSFSC scores around 3.3 A for data that is clearly closer to 6 A. Apologies for the possible naivety, but I did not find a post covering this topic.

2

What does the movie_blob/shape line look like in import movies?

3

mscope_params/accel_kv : {300.0}
mscope_params/cs_mm : {0.1}
mscope_params/total_dose_e_per_A2 : {45.03}
mscope_params/exp_group_id : {22}
mscope_params/phase_plate : {0}
mscope_params/neg_stain : {0}
movie_blob/psize_A : {0.557}
movie_blob/shape : [ 30 3710 3838]

4

Not super res… double check that you are importing the correct movies, or that super res was set correctly during data collection

5

That was rather straightforward… thank you very much Oli

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