I imported my particle images after crYOLO picking and made 2D classifications. Then after the first refinement I subtracted one part of the structure and continued with local refinement. The end result (the aligned subtracted particles) was exported to a star file to make local 3D classifications in Relion without alignment. Now, after selecting the interesting class in Relion, I would like to do a new local refinement with a subset of particles in CryoSparc, but I don’t know how to do that.
I tried to import the subset star file of the subtracted particles, but I lose the (original CryoSparc) alignment parameters. Unfortunately, I don’t know how to use only a subset in CryoSparc or to relate the subtracted particle set to the original one to redo the full procedure from the beginning but only with the subset.
Any help would be appreciated.