Strategy for membrane protein in a very tight nanodisc

glad to hear it! :slight_smile: Yep keep going until things start getting worse. You may want to vary the decoy models you use, too - sometimes I find it helps to generate decoys by running ab initio on discarded particles (to generate data-driven junk models)

Also once the resolution improves a bit, you may want to revisit local refinement (with or without signal subtraction) - it often gives a considerable improvement in such cases.

Cheers
Oli

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