Strange image corruption during cryosparc live .mrc import

CryoSPARC Live
1

Dear cryosparc team,

I had a strange image “corruption” during cryosparc live:

image
image1920×1160 227 KB

Raw images were checked in 3dmod and look ok.

Best regards,
Nuno

2

Would you be willing to share one of the “offending” movies with us, along with

  • a screenshot the corresponding pre-processed image
  • a screenshot of the configuration tab (pixel size, spherical aberration, dose, voltage, …)
  • gain reference

?
If needed, we can provide a link for data upload.

3

Of course I can share it. I imported directly motion corrected file (.mrc)?

Does this change your files request?

Best regards,
Nuno

4

CryoSPARC Live requires the import of movies. The import of motion-corrected, single-frame micrographs is not supported.

Thank you for providing the additional details, which fully explain the problem. That removes the need for sharing sample files with us.

5

It worked for 5000 images

6

Please can you try importing the motion-corrected mrc files into regular CryoSPARC, and report errors you observe during downstream processing in regular CryoSPARC.

7

That’s what I did afterwards. I didn’t have any problem.

The downstream processing in regular CryoSPARC went smoothly.

Best,
Nuno