I am trying to solve a structure of a complex which behaves like a spiral hexamer probably not much is known about it. I would like to know are there specific parameters which I should be aware of in 2d classification or further downstream processing? Kindly shed some of your opinion it will be really helpful
Hi,
When you say spiral hexamer, is it a repetitive one? If yes you need to follow the helical reconstruction parameters starting from particle picking to refinement.
Best.
Hi,
It would be best to determine a workflow based on the particle behaviour and distribution you see in negative stain or screened grids. It sounds like it might require helical reconstruction pipeline as suggested by others, but no need to rule out SPA too quickly. Hope it works out.
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hii
it is spiral of repititive subunit. can you share some link where i can get pipeline of helical reconstruction?