Size of 3D volume to generate templates

Greeting there,

To generate 2D templates, the imported 3D volume has to be equal at x and y or?

Thanks in advance for any input,

Probably yes. If you are generating templates from a molmap volume in ChimeraX you should use the ongrid #N option to generate the simulated map on the same grid as one of your reconstructions. You can also use some vop subcommands to make a new one of a certain size and pixel spacing.