Signal subtraction does not subtract

Hi, I have a complex which is hexamer but I want to do focus classification at monomeric level so I used the expand symmetry and a 5monomer mask and the C6 map for signal subtraction but when I do 2D classification on subtracted particles it seems that it wasn’t done any subtraction on particles and still it shows the full hexamer. Does anyone know what is the problem?

Hi @sepidehv,

Any chance you could provide some screenshots? Please email
if you are unable to post them publicly on the forum.