Signal subtract, align, then revert to un-subtract particles



hi, is it possible to do subtraction, then align, then swap out the particles so that I have my unsubtracted particles using my alignments from the subtracted ones? The reason is that I have density that is messing up alignment. I want to remove it just to get the alignment of the core, then revert back so I can see how the subtracted areas look once I’ve aligned correctly on the core.



Hi @orangeboomerang,

Unfortunately there is no way to directly do this with the current functionality in cryoSPARC. Thank you for pointing this out, it would be a very useful tool and we’ll add it to a list of possible future improvements.