Sometimes, when one orientation of a particle is rare or low contrast (e.g. top views vs side views), I would like to process it separately from the bulk, by selecting these 2D classes and then performing subclassifications on e.g. side and top views separately.
I thought a convenient way to do this (and to make sure that the same particle doesn’t get assigned to more than one group, and that no good 2D classes are missed), would be to perform an initial select2D job, where for example side view classes are selected, then take the templates_excluded and particles_excluded outputs from this job and use them to start a second Select2D job (where top view classes will be selected).
However, when I do this the second Select2D job has class assignments that don’t make any sense - the thumbnails corresponding to previously highly occupied classes (that weren’t selected in the first round) have 0 particles, and those that were previously minor junk classes with few particles now have many particles assigned. So I suspect that somehow cryosparc is displaying the wrong thumbnail for each class in this case. Possible bug?