Hi,
My sample is long filaments entire length around 2.3 micron, and we usually reconstruct 5 crown (which is 725A), so we pick a box size that can fit 725A. we are actually picking segments of filaments instead of entire filament. When I use the filament tracer to pick the particles (in this case segments), I space them like 1 crown (145A) which is the separation between picks in terms of diameter in the filament tracer job, so I put 0.725 coz my particle diameter is 200A, so if I want it to place each box around 145A spacing, it is 0.725 (in terms of diameter).
Which is not leaving out the neighboring particles that might be sitting right next to each other. Since I am reconstructing 725A worth of spacing of the filament, and I am only separating them at 145A, along the filament there’s multiple picks in same filament, like distance between one box center to next box center is 145A, and my reconstruction is 725A worth of space, so there’s definitely repeat, I wish I could only pick particles that are 725A apart that means no repeat in 5 crown. But when I set the separation parameter spacing as 725A, it doesn’t consider 725A distance in one direction, along the same filament, it considers 725A radially, like a big circle so the neighboring filament is not getting counted even if they are part of another filament.
So my question, is there any way I can pick filament segments specifying that I want each box to be placed at 725A distance from each other along the filament instead of considering that distance radially which excluding my neighboring filaments which might just sitting in close proximity?
If Cryosparc don’t have that feature, is there any other filament picking software, where I can direct the software to pick in every 725A distance in one direction (along the filament length) instead of considering 725A distance in all direction?
