Hi all,
I’m writing a guide for my lab on how to get cryosparc extracted particles into RELION. I managed to get it working, but I wanted to know what exactly each restacked particle and passthrough file is doing. So far, I’ve gotten 2D classification to work with the particles and all passthrough files except for 2D and 3D alignments.
I’m tempted to just get people to include all 6 files when converting to star, but first I wanted to understand what exactly each of these .cs files are storing. Thanks!