Request for Manual Picker to output extracted particles and selected particles

Application
1

Hello,

The Manual Picking job currently outputs only the extracted particles as defined by the ‘box size’ option in the interactive Manual Picker window. I would like to request that all coordinates for all selected particles be outputted as well. This will allow the user to re-define the box size after Manual Picking without losing picks. I realized that this feature would be nice by accidentally having a box size of 1500 when I finished manual curating 300 micrographs because I was removing all picks in the last micrograph quickly. This resulted in only ~10% of the particles I just curated being extracted; ie. I wasted a few hours of work.

Thank you,
-Alex

2

Hey @alexjamesnoble ,

Thanks for reporting! Along with making extraction an optional step, we’ll make sure there’s a set of particle locations outputted that don’t exclude particles at the edge of the micrograph (a function of the box size that was selected) so you can always re-extract all the particles you selected with different parameters.

- Suhail

1 Like