After running homo refinement, I did the remove duplicates to check if there is any duplicates in my final dataset. It turned out that remove duplicates remove particles that are clearly not duplicates. The result is attached. Does anyone have an idea what could cause this problem?
I set the minimum separation distance to 20Å, shift key to alignment3D and scored field to pick_stats/ncc_score. The diameter of the white circle is ~250Å.
The alignments 3D originated from Homo refinement with re-extracted unbinned particles.
I’ve noticed that when I ran remove duplicates after Homo refinement with binned particles (bin4), without re-extraction, remove duplicates yields reasonable results. It appears that something went wrong during the re-extraction process.
@Ty22 Thanks for your post. Please let us know if you would be willing to share some data with the Structura team so that we may arrangements a transfer of the following data:
From the job directory of the homogeneous refinement job:
JX_{last_iteration_number}_particles.cs
JX_particles.csg
JX_passthrough_particles.cs
From the job directory of the upstream Extract from Micrographs job that the particles came from (the problematic re-extraction job):