I have been processing this data for several months and decided to go back to a particular stack to do some quantification. I tried to run a remove duplicates job and received the following error:
Traceback (most recent call last):
File "cryosparc_master/cryosparc_compute/run.py", line 52, in cryosparc_compute.run.main
File "/admin/opt/common/cryosparc3.1/cryosparc_master/cryosparc_compute/jobs/runcommon.py", line 838, in check_default_inputs
assert False, 'Non-optional inputs from the following input groups and their slots are not connected: ' + missing_inputs + '. Please connect all required inputs.'
AssertionError: Non-optional inputs from the following input groups and their slots are not connected: particles.location. Please connect all required inputs.
I tried this both with and without micrographs provided as inputs (providing pixel size instead). Thinking it may have been the particular particle stack, I went to another stack and tried the same thing to no avail. Am I missing something here?
J3361 is a Remove Duplicates job. I’m looking for particle location meta data in the low level jobs and only Extract or Particle Sets jobs, as far as I can tell, contain this link. Strangely, Import jobs do not. This particle stack was imported from a Relion Polish job. Also, I can successfully run a Local Refinement on this stack prior to the Remove Duplicates job. I’m a little stumped.