Hello all,
I have a potentially silly question and I hope someone might be able to help me understand.
Imagine during 3D classification you get 10 classes, each of which have their own symmetry candidate. These candidates are all similarly resolved to 5A and the candidate twist values vary from 100 degrees to 105 degrees. You might think, great! I have identified some level of heterogeneity in the data!
But is this true? How can we calculate what the minimum difference in helical twist value observable at a given resolution is? If your particles all fall within a range lower than this theoretical limit, do we have to accept that we only approximating the true twist angle, at least until resolution improves?
This is perhaps a stupid question - but it would be great to know how best to think about this.
Best regards,
Charlie