Reference-based motion correction with symmetry-expansion

[olibclarke]

@rbs_sci

Next on the wishlist; RBMC of symmetry expanded data?

Yes please! Preferably (IMO) with an option to use the full particle for alignment, then extract sym-expanded subparticles from the aligned frames:

Local motion correction of subparticles?

Hi, What is the best workflow to perform local motion correction of subparticles (either reference-based or otherwise)? Ideally, I would like to calculate trajectories from the full particle (which has more signal, and more stable trajectories), but then extract matched sets of subparticles using the improved local alignment parameters and dose weighting. Currently, the best way I can think of to do this is to align/extract in an oversized box, reconstruct, then use volume alignment tools to …