Reference Based Motion Correction vs Local motion correction

Dear CryoSPARC team,

Great work on a massive update! I have an immediate question: what are the differences between Reference Based Motion Correction vs Local motion correction? Will either one be sufficient for enough improvement? or Reference based motion correction will be better than local motion correction? Currently I always use local motion correction for my final round of processing, and routinely get ~0.2A improvement.

Thank you!

Hi @parrot!

Generally, if speed is not your number one priority, we recommend using Reference Based Motion Correction, since it is the only job which provides empirical does weights. We have found that empirical dose weights provide significant benefit to final reconstruction quality, even when Local Motion Correction provides similar estimates on particle motion.

Thank you for your reply! I will test both to see how much time the Reference based motion correction job will take.