Reference based motion correction output no trajectory

Motion Correction
1

I am processing super-resolution movies collected on K3. After binning, the box size is 480. Following NU-refinement, I applied reference-based motion correction, which resulted in a box size of 960 with normal trajectories.

J18_
J18_551×406 30.7 KB

However, when I used Fourier cropping in the reference-based motion correction to revert the box size back to 480, no trajectories were produced. It appears that the particles are somehow immobilized.

J22_
J22_551×406 14.4 KB

Any suggestions would be appreciated!

2

That’s curious, never seen that before… will be interested in hearing what the devs think.

That said, I would recommend (if you’re close to binned Nyquist and you’ve got the RAM) to RBMC to full size (i.e. 960 pixel box as above) and then downsampling the output using a separate job. That way, if you’re closer to Nyquist, you can adjust the downsampling parameters to taste. :wink:

3

I’ll try that.
Thanks!