RBMC protein size limit?


I am currently processing a data set of a globular ~65 kDal protein and trying to use RBMC to improve resolution. The hyperparameter search shows promising results:



However, when running the motion correct particles step the particles lack trajectories:


Using these particles in a subsequent refinement results in a worsening of the overall resolution compared to a non-RBMC particle stack.

We have run RBMC on larger proteins and it works well. Is there potential parameter tweak or work around for smaller proteins?

See here for fix, which so far had medium success for me.

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