I am currently processing a data set of a globular ~65 kDal protein and trying to use RBMC to improve resolution. The hyperparameter search shows promising results:
However, when running the motion correct particles step the particles lack trajectories:
Using these particles in a subsequent refinement results in a worsening of the overall resolution compared to a non-RBMC particle stack.
We have run RBMC on larger proteins and it works well. Is there potential parameter tweak or work around for smaller proteins?