RBMC protein size limit?

[bdcook]

Hello,

I am currently processing a data set of a globular ~65 kDal protein and trying to use RBMC to improve resolution. The hyperparameter search shows promising results:

However, when running the motion correct particles step the particles lack trajectories:

Using these particles in a subsequent refinement results in a worsening of the overall resolution compared to a non-RBMC particle stack.

We have run RBMC on larger proteins and it works well. Is there potential parameter tweak or work around for smaller proteins?

[CryoEM2]

RBMC zero motion

it did say so: Exiting early, all search paths have reached the ~zero trajectory regime.

See here for fix, which so far had medium success for me.