Dear All,
I know relion and cryosparc have different conventions for Dn symmetry and we need to transform the cs file with pyem before we import Dn particles from Cryosparc into Relion. But I wonder if we need to transform the star file before we import Dn particles from Relion into Cryosparc. It seems that the reconstruction in Cryosparc has no obvious errors even if no transformation is applied to the star file before I import them from relion into cryosparc. Does Cryosparc do the transformation automatically when importing Dn particles from Relion? Thank you for your answers.