I have a somewhat basic question: when Ref-based motion correction is performed does cryosparc go back to the imported .eer files or to the original Motion Correction job after the import. I am asking as both directories (the raw .eer files and the MoCor output) take about the same huge space so it would be great if we can remove safely the raw data to free up space for downstream processing and more datasets.
You certainly need the raw movie files and gain reference (.eer and .gain).
I’m not 100% sure whether RBMC reads the rough trajectories precalculated by Patch Motion or not; I don’t think it does… but @hsnyder will know for sure and correct me if I’m wrong.
