Post symmetry expansion and local refinement

Local Refinement
1

Hi folks,

After symmetry expansion (C7) and local refinement with mask on just single subunit I have an excellent single subunit map. How do I apply the symmetry (C7) back to the map to make it complete? Right now I just have a single subunit map and I want it to be a complete C7 ring.

Any thoughts or ideas are appreciated.

Thank you

Prash

2

Align seven copies with your overall C7 reconstruction, then combine (vop maximum in Chimera) and apply C7 symmetry (e.g. using relion_image_handler)?

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3

This worked!
Thank you @olibclarke, you are a genius!

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