Poor 2d class average

What’s the mass of the particle? Can you clearly see particles in your micrographs, and are they being picked well?

If the particle is on the smaller side (<100 kDa) or has low SNR, then turning force max poses over shifts off might be helpful. You should set around 80 online-EM iterations, because the job will take a lot longer to converge. This post has some helpful information on what parameters to choose:

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