Pixel tweak in Sharpening?

Post Processing
1

I can adjust pixel size in DeepEMhancer and Relion postprocessing. Is there a way to do this in Sharpening tools or similar, as this generates the preferred map for model building?

2

Hi @CryoEM2,

I believe in the “Validation (FSC)” job, you are able to adjust the pixel size by inputting a new one as a parameter. The job will then compute the FSC given the corrected pixel size, and output the raw half-maps also with the corrected pixel size. These can then be passed to a Sharpening job to generate a sharpened map with the right pixel size.

Best,
Michael

1 Like
3

thank you, works perfectly!