Patch motion override dose query

Motion Correction
1

Hi,

In patch motion, there is an option to override the total dose (e.g. if it was wrong at movie import).

My question is, if one uses a range of frames less than the total, does cryosparc account for that? Or does it still split the total dose assigned at import across the new, smaller frame range, in which case it might make sense to override in this case?

Cheers
Oli

2

Hi Oli,

CryoSPARC takes the dose (either the original or the override, if the latter is supplied) and divides it by the number of frames in the raw movie. So, if you’re only using a subset of the frames for the actual algorithm, the dose per frame will be unaffected.

– Harris

1 Like