I have refined maps to ~3.7-4A resolution in cryosparc using imported particles and micrographs from Relion3.1. The micrographs were motion-corrected using MotionCor2 and the CTFs were estimated with ctffind4.1. I have read the tutorial on Local CTF refinement, Patch CTF Estimation and Patch CTF Extraction but unsure if I understand it correctly.
Can I use the following procedure to check if cryosparc CTFs improve my refinement?
- Run Patch CTF Estimation using micrographs from the last Particle Extraction in cryosparc as input
- Run Patch CTF Extraction using the output from #1 above and particles from NU refinement as input.
- Run particles from #2 above as input to a new NU refine job with volume from old NU refine as reference