I wanted to give a quick update on what sort of “worked” or didn’t for me:
(i) overpicking using DogPicker, followed by 2D classification. Then, template picking in RELION
(ii) template picking with cryoSPARC does not seem to work for this data set…
Ab into model generation
(i) RELION didn’t work
(ii) Imported particles from RELION 2d classification into cryoSAPARC and generated ab initio models. cryoSPARC was able to give two separate conformations
(i) One conformation refined to ~ 5A and the other to ~ 7A
I would like to ask the cryoSPARC community what the best way is to use these models to extract more particles from the micrographs ?
Generate templates from the 3D volumes and use those for particle picking ?
sort particles in these volumes by 2d classification and use the 2d classes as templates?
Thanks a lot.