Hello,
Using external libraries and cryosparc-tools, I am looking to create a series of 2d projections of the 3d difference map of two volumes. I have the main portion of the code down, but am curious as to how the center of rotation is defined.
Specifically, for a given volume, I can find either:
i) The center of the box
ii) The center of mass of the volume (ChimeraX->Tools->Actions->Volume Data->Surface Color->Clicking center
For example, these two methods would yield coordinates of (196, 196, 196) or (287.62, 286.15, 286.27). If now I want to rotate the volume to a given orientation (defined by azimuth and elevation) obtained from -for example- the maximum of a orientation distribution plot. Would I rotate my volume from the center of the box or the center of mass of the volume?