Orientation Sorting Tools

Features and Functionality
1

Maybe requested before, maybe a bit of a long shot…

I think it could be super useful to have a little GUI/interactive job that makes the input job’s Viewing Direction Distribution interactive. You could draw a little polygon on it, then it would output particles from within that polygon (and I guess an excluded category too).

I think this could be helpful in a few ways. You could confirm which views populate which “hot-spots” for over representation in your plots, thus assisting in attempts to re-balance. As well, you could look specifically at less well represented regions, do downstream 2Ds, pipe this into Topaz and try and boost these views, etc.

Regardless, thanks devs for the great software.

Cheers,
NAC

3 Likes
2

You can also use star2bild.py to make Relion-style distribution plots for ChimeraX and then simply look at what views/parts of the structure are sticking in 3D.

2 Likes
3

+1 to this - it seems like rebalancing views based on 3d classification of particles would be much more efficient than using 2D views, especially when trying to find rare 2D views

2 Likes
4

Looks like Sami Chaaban made this for STAR files. Would still love to see this sort of thing incorporated into CryoSPARC.

https://twitter.com/sami_chaaban/status/1695508724637405354?s=20

1 Like