Non-uniform Refinement interpretation

Cryo-EM Data Processing
1

Hi all,

I need some insight for the interpretation of nu-refinement results.

J864_real_space_slices_iteration_007.png
J864_real_space_slices_iteration_007.png1090×341 120 KB

What does represent this color panel ? The protein I study exposed a C2 symmetry, here with a 230 pix box. During the first steps of processing, this panel was blue with a C1 symmetry and a 160 pix box. No mask was used for this refinement.

Thanks for your help!
Kevin

2

They are 3 orthogonal central slices through the map.

Perhaps you didn’t have your reference aligned to the symmetry axis correctly (3rd panel on right should have C2 symmetry evident) initially, but the particle became correctly aligned by chance after symmetry was enforced.