Hello,
I am using CryoSPARC v4.2.0 and I get the error:
Job is unresponsive - no heartbeat received in 180 seconds
Small jobs run fine such as importing movies, but when I come to do patch motion correction the job fails at anywhere between 100-500 micrographs. Only once did the PMC complete 1,000 micrographs. It is impossible to process a large amount of data meaning that I am at a dead end. Does anyone have any advice or experience with this issue? Is it caused by the way CryoSPARC is perhaps is set up?
I have put the event log below:
[CPU: 367.9 MB Avail: 249.92 GB]
--------------------------------------------------------------
[CPU: 367.9 MB Avail: 249.92 GB]
Processed 500 of 3157 movies in 5109.30s
[CPU: 1.95 GB Avail: 251.21 GB]
-- 0.0: processing 503 of 3157: J73/imported/011536600233019576460_FoilHole_22123076_Data_22074515_22074517_20230225_213212_Fractions.mrc
loading /opt/cryosparc/cryosparc_worker/cryosparc_compute/CS-rrp2-glacios/CS-rrp2-glacios-ii/J73/imported/011536600233019576460_FoilHole_22123076_Data_22074515_22074517_20230225_213212_Fractions.mrc
Loading raw movie data from J73/imported/011536600233019576460_FoilHole_22123076_Data_22074515_22074517_20230225_213212_Fractions.mrc ...
Done in 6.26s
Processing ...
Done in 2.75s
Completed rigid and patch motion with (Z:4,Y:6,X:6) knots
Writing non-dose-weighted result to J79/motioncorrected/011536600233019576460_FoilHole_22123076_Data_22074515_22074517_20230225_213212_Fractions_patch_aligned.mrc ...
Done in 0.03s
Writing 120x120 micrograph thumbnail to J79/thumbnails/011536600233019576460_FoilHole_22123076_Data_22074515_22074517_20230225_213212_Fractions_thumb_@1x.png ...
Done in 0.00s
Writing 240x240 micrograph thumbnail to J79/thumbnails/011536600233019576460_FoilHole_22123076_Data_22074515_22074517_20230225_213212_Fractions_thumb_@2x.png ...
Done in 0.00s
Writing dose-weighted result to J79/motioncorrected/011536600233019576460_FoilHole_22123076_Data_22074515_22074517_20230225_213212_Fractions_patch_aligned_doseweighted.mrc ...
Done in 0.04s
Writing background estimate to J79/motioncorrected/011536600233019576460_FoilHole_22123076_Data_22074515_22074517_20230225_213212_Fractions_background.mrc ...
Done in 0.00s
Writing motion estimates...
Done in 0.00s
[CPU: 3.23 GB Avail: 249.95 GB]
-- 0.0: processing 504 of 3157: J73/imported/016050821538335556846_FoilHole_22123077_Data_22074515_22074517_20230225_213043_Fractions.mrc
loading /opt/cryosparc/cryosparc_worker/cryosparc_compute/CS-rrp2-glacios/CS-rrp2-glacios-ii/J73/imported/016050821538335556846_FoilHole_22123077_Data_22074515_22074517_20230225_213043_Fractions.mrc
Loading raw movie data from J73/imported/016050821538335556846_FoilHole_22123077_Data_22074515_22074517_20230225_213043_Fractions.mrc ...
Done in 6.23s
Processing ...
Done in 2.74s
Completed rigid and patch motion with (Z:4,Y:6,X:6) knots
Writing non-dose-weighted result to J79/motioncorrected/016050821538335556846_FoilHole_22123077_Data_22074515_22074517_20230225_213043_Fractions_patch_aligned.mrc ...
Done in 0.03s
Writing 120x120 micrograph thumbnail to J79/thumbnails/016050821538335556846_FoilHole_22123077_Data_22074515_22074517_20230225_213043_Fractions_thumb_@1x.png ...
Done in 0.00s
Writing 240x240 micrograph thumbnail to J79/thumbnails/016050821538335556846_FoilHole_22123077_Data_22074515_22074517_20230225_213043_Fractions_thumb_@2x.png ...
Done in 0.00s
Writing dose-weighted result to J79/motioncorrected/016050821538335556846_FoilHole_22123077_Data_22074515_22074517_20230225_213043_Fractions_patch_aligned_doseweighted.mrc ...
Done in 0.03s
Writing background estimate to J79/motioncorrected/016050821538335556846_FoilHole_22123077_Data_22074515_22074517_20230225_213043_Fractions_background.mrc ...
Done in 0.00s
Writing motion estimates...
Done in 0.00s
[CPU: 1.92 GB Avail: 251.25 GB]
-- 0.0: processing 505 of 3157: J73/imported/002847857095426660098_FoilHole_22123080_Data_22074515_22074517_20230225_215711_Fractions.mrc
loading /opt/cryosparc/cryosparc_worker/cryosparc_compute/CS-rrp2-glacios/CS-rrp2-glacios-ii/J73/imported/002847857095426660098_FoilHole_22123080_Data_22074515_22074517_20230225_215711_Fractions.mrc
Loading raw movie data from J73/imported/002847857095426660098_FoilHole_22123080_Data_22074515_22074517_20230225_215711_Fractions.mrc ...
Done in 6.39s
Processing ...
Done in 2.68s
Completed rigid and patch motion with (Z:4,Y:6,X:6) knots
Writing non-dose-weighted result to J79/motioncorrected/002847857095426660098_FoilHole_22123080_Data_22074515_22074517_20230225_215711_Fractions_patch_aligned.mrc ...
Done in 0.03s
Writing 120x120 micrograph thumbnail to J79/thumbnails/002847857095426660098_FoilHole_22123080_Data_22074515_22074517_20230225_215711_Fractions_thumb_@1x.png ...
Done in 0.00s
Writing 240x240 micrograph thumbnail to J79/thumbnails/002847857095426660098_FoilHole_22123080_Data_22074515_22074517_20230225_215711_Fractions_thumb_@2x.png ...
Done in 0.00s
Writing dose-weighted result to J79/motioncorrected/002847857095426660098_FoilHole_22123080_Data_22074515_22074517_20230225_215711_Fractions_patch_aligned_doseweighted.mrc ...
Done in 0.03s
Writing background estimate to J79/motioncorrected/002847857095426660098_FoilHole_22123080_Data_22074515_22074517_20230225_215711_Fractions_background.mrc ...
Done in 0.00s
Writing motion estimates...
Done in 0.00s
[CPU: 3.23 GB Avail: 249.95 GB]
-- 0.0: processing 506 of 3157: J73/imported/004062294440860625095_FoilHole_22123081_Data_22074515_22074517_20230225_215145_Fractions.mrc
loading /opt/cryosparc/cryosparc_worker/cryosparc_compute/CS-rrp2-glacios/CS-rrp2-glacios-ii/J73/imported/004062294440860625095_FoilHole_22123081_Data_22074515_22074517_20230225_215145_Fractions.mrc
Loading raw movie data from J73/imported/004062294440860625095_FoilHole_22123081_Data_22074515_22074517_20230225_215145_Fractions.mrc ...
Done in 6.88s
Processing ...
Done in 2.66s
Completed rigid and patch motion with (Z:4,Y:6,X:6) knots
Writing non-dose-weighted result to J79/motioncorrected/004062294440860625095_FoilHole_22123081_Data_22074515_22074517_20230225_215145_Fractions_patch_aligned.mrc ...
Done in 0.03s
Writing 120x120 micrograph thumbnail to J79/thumbnails/004062294440860625095_FoilHole_22123081_Data_22074515_22074517_20230225_215145_Fractions_thumb_@1x.png ...
Done in 0.00s
Writing 240x240 micrograph thumbnail to J79/thumbnails/004062294440860625095_FoilHole_22123081_Data_22074515_22074517_20230225_215145_Fractions_thumb_@2x.png ...
Done in 0.00s
Writing dose-weighted result to J79/motioncorrected/004062294440860625095_FoilHole_22123081_Data_22074515_22074517_20230225_215145_Fractions_patch_aligned_doseweighted.mrc ...
Done in 0.03s
Writing background estimate to J79/motioncorrected/004062294440860625095_FoilHole_22123081_Data_22074515_22074517_20230225_215145_Fractions_background.mrc ...
Done in 0.00s
Writing motion estimates...
Done in 0.00s
[CPU: 1.92 GB Avail: 251.26 GB]
-- 0.0: processing 507 of 3157: J73/imported/002500836666533175262_FoilHole_22123082_Data_22074515_22074517_20230225_215159_Fractions.mrc
loading /opt/cryosparc/cryosparc_worker/cryosparc_compute/CS-rrp2-glacios/CS-rrp2-glacios-ii/J73/imported/002500836666533175262_FoilHole_22123082_Data_22074515_22074517_20230225_215159_Fractions.mrc
Loading raw movie data from J73/imported/002500836666533175262_FoilHole_22123082_Data_22074515_22074517_20230225_215159_Fractions.mrc ...
Done in 6.54s
Processing ...
Done in 2.62s
Completed rigid and patch motion with (Z:4,Y:6,X:6) knots
Writing non-dose-weighted result to J79/motioncorrected/002500836666533175262_FoilHole_22123082_Data_22074515_22074517_20230225_215159_Fractions_patch_aligned.mrc ...
Done in 0.03s
Writing 120x120 micrograph thumbnail to J79/thumbnails/002500836666533175262_FoilHole_22123082_Data_22074515_22074517_20230225_215159_Fractions_thumb_@1x.png ...
Done in 0.00s
Writing 240x240 micrograph thumbnail to J79/thumbnails/002500836666533175262_FoilHole_22123082_Data_22074515_22074517_20230225_215159_Fractions_thumb_@2x.png ...
Done in 0.00s
Writing dose-weighted result to J79/motioncorrected/002500836666533175262_FoilHole_22123082_Data_22074515_22074517_20230225_215159_Fractions_patch_aligned_doseweighted.mrc ...
Done in 0.03s
Writing background estimate to J79/motioncorrected/002500836666533175262_FoilHole_22123082_Data_22074515_22074517_20230225_215159_Fractions_background.mrc ...
Done in 0.00s
Writing motion estimates...
Done in 0.00s
[CPU: 3.23 GB Avail: 249.95 GB]
-- 0.0: processing 508 of 3157: J73/imported/011861353430844636542_FoilHole_22123083_Data_22074515_22074517_20230225_215216_Fractions.mrc
loading /opt/cryosparc/cryosparc_worker/cryosparc_compute/CS-rrp2-glacios/CS-rrp2-glacios-ii/J73/imported/011861353430844636542_FoilHole_22123083_Data_22074515_22074517_20230225_215216_Fractions.mrc
Loading raw movie data from J73/imported/011861353430844636542_FoilHole_22123083_Data_22074515_22074517_20230225_215216_Fractions.mrc ...
Done in 6.54s
Processing ...
Done in 2.62s
Completed rigid and patch motion with (Z:4,Y:6,X:6) knots
Writing non-dose-weighted result to J79/motioncorrected/011861353430844636542_FoilHole_22123083_Data_22074515_22074517_20230225_215216_Fractions_patch_aligned.mrc ...
Done in 0.03s
Writing 120x120 micrograph thumbnail to J79/thumbnails/011861353430844636542_FoilHole_22123083_Data_22074515_22074517_20230225_215216_Fractions_thumb_@1x.png ...
Done in 0.00s
Writing 240x240 micrograph thumbnail to J79/thumbnails/011861353430844636542_FoilHole_22123083_Data_22074515_22074517_20230225_215216_Fractions_thumb_@2x.png ...
Done in 0.00s
Writing dose-weighted result to J79/motioncorrected/011861353430844636542_FoilHole_22123083_Data_22074515_22074517_20230225_215216_Fractions_patch_aligned_doseweighted.mrc ...
Done in 0.03s
Writing background estimate to J79/motioncorrected/011861353430844636542_FoilHole_22123083_Data_22074515_22074517_20230225_215216_Fractions_background.mrc ...
Done in 0.00s
Writing motion estimates...
Done in 0.00s
[CPU: 1.95 GB Avail: 251.20 GB]
-- 0.0: processing 509 of 3157: J73/imported/007513752052181863553_FoilHole_22123084_Data_22074515_22074517_20230225_215228_Fractions.mrc
loading /opt/cryosparc/cryosparc_worker/cryosparc_compute/CS-rrp2-glacios/CS-rrp2-glacios-ii/J73/imported/007513752052181863553_FoilHole_22123084_Data_22074515_22074517_20230225_215228_Fractions.mrc
Loading raw movie data from J73/imported/007513752052181863553_FoilHole_22123084_Data_22074515_22074517_20230225_215228_Fractions.mrc ...
Done in 6.59s
Processing ...
Done in 2.58s
Completed rigid and patch motion with (Z:4,Y:6,X:6) knots
Writing non-dose-weighted result to J79/motioncorrected/007513752052181863553_FoilHole_22123084_Data_22074515_22074517_20230225_215228_Fractions_patch_aligned.mrc ...
Done in 0.03s
Writing 120x120 micrograph thumbnail to J79/thumbnails/007513752052181863553_FoilHole_22123084_Data_22074515_22074517_20230225_215228_Fractions_thumb_@1x.png ...
Done in 0.00s
Writing 240x240 micrograph thumbnail to J79/thumbnails/007513752052181863553_FoilHole_22123084_Data_22074515_22074517_20230225_215228_Fractions_thumb_@2x.png ...
Done in 0.00s
Writing dose-weighted result to J79/motioncorrected/007513752052181863553_FoilHole_22123084_Data_22074515_22074517_20230225_215228_Fractions_patch_aligned_doseweighted.mrc ...
Done in 0.03s
Writing background estimate to J79/motioncorrected/007513752052181863553_FoilHole_22123084_Data_22074515_22074517_20230225_215228_Fractions_background.mrc ...
Done in 0.00s
Writing motion estimates...
Done in 0.00s
[CPU: 3.23 GB Avail: 249.93 GB]
-- 0.0: processing 510 of 3157: J73/imported/002167456673077019549_FoilHole_22123085_Data_22074515_22074517_20230225_215241_Fractions.mrc
loading /opt/cryosparc/cryosparc_worker/cryosparc_compute/CS-rrp2-glacios/CS-rrp2-glacios-ii/J73/imported/002167456673077019549_FoilHole_22123085_Data_22074515_22074517_20230225_215241_Fractions.mrc
Loading raw movie data from J73/imported/002167456673077019549_FoilHole_22123085_Data_22074515_22074517_20230225_215241_Fractions.mrc ...
Done in 6.61s
Processing ...
Job is unresponsive - no heartbeat received in 180 seconds.
Many thanks in advance!
Chloe