Negative stain blob picker wrong

Particle Picking
1

Hi，

I’m trying to process negative stain data. After I imported my mrc files ( toogled as negative data), I did a blob picker job. But all target particles (white particles) were not picked. Do I have to invert my mrc files’ contrast before import into cryosparc? Is there a more convenient way？

Best，
Wendy

2

@Wendy Which version of CryoSPARC do you use?

3

My cryosparc version is v4.3.0.

Thanks.
Wendy

4

Thanks @Wendy for reporting the issue. We have recorded it.

5

Hi Wendy,
I had the same problem when processing negative data where there was a strong black halo around the particle and where the stain was too thin. It seemed to pick correctly on grids where the background was significantly darker than the overall particle.

1 Like
6

Manual pick 20, 2D class 2 classes, use good template to
Template Pick