Hello everyone:)
I’ve encountered an issue with Patch CTF Estimation on my processing and I could really use some advice.
Recently, we had a pixel size adjustment on our Glacios microscope(with Falcon 4i) from the original 0.8939 to 0.841(mag → 120k). I successfully processed a batch of 200 apoferritin images with the new pixel size without any issues. However, when I attempted to process 2000 images of my protein sample using the updated pixel size, I ran into an NaN/Inf error at the Patch CTF Estimation step today (screenshot).
Interestingly, when I reverted back to the old pixel size of 0.8939 for the protein sample, the processing went through without any errors. I didn’t change any other parameter changes except pixel size… then the cause of this issue would likely be the pixel size, right…? Is it possible that the pixel size isn’t the cause? This issue wouldn’t have occurred because processing was done with files uploaded to the server while image acquisition was still ongoing, would it?
If new pixel size was wrong, why there were no issues when processing the apoferritin images with the new pixel size? I am just so confuse… Could there be a specific reason why the pixel size change would affect one sample and not the other, or could it just be a coincidence?
If my question sounds a bit silly, I apologize…