Hi, I am knew to CryoEM and trying to reconstruct images for alpha-synuclein fibrils from EMPIAR-10195 . Micrographs have been already drift-corrected and dose-weighted using MotionCor2.
After I imported the exposures to CryoSparc, I ran CTF estimation and now am at the exposure curation job. When looking at the thon rings image, there are many other thon rings overlapped on the main one (look at picture). Should I be concerned? What does it mean?