For our K3 data, we need to do the Distortion correction in the motioncor2 to obtain the right astigmatism evaluation.
But I don’t know how to do it in cryoSPARC.
Motioncor2 has the -Mag option to correct the Distortion.
- Correct anisotropic magnification by stretching
image along the major axis, the axis where the
lower magificantion is detected.
- Three inputs are needed including magnifications
along major and minor axes and the angle of the
major axis relative to the image x-axis in degree.
- By default no correction is performed.