Motion corrected micrographs from Relion 3.1.3

Yes, 2000 good particles is plenty for training.

Re filament picking, crYOLO has an excellent filament picker which might be worth a go. I don’t think helical refinement will help you here, as it doesn’t seem to be a helical assembly.

Good luck!

Cheers
Oli

Hi Oli,

Thanks for your help. Let’s see how it goes.