Metadata path .star file

2D Classification
1

I have 10000 2D images stack:mrcfile . how can I write the .star file for building the metadata? (new to cryosparc)

2

https://github.com/asarnow/pyem

But if you download the .mrc particle stack form EMPIAR it can be imported directly to cryosparc.

The metadata for cryosparc (.cs) and RELION (.star) need conversion. Particles + .star metadata also work in other SPA programs.

3

I am trying to write the .star file on my own, using the following
with open(output_path, ‘w’) as star_file:
star_file.write(‘\ndata_\n\nloop_\n’)
star_file.write(‘_rlnImageName #1\n’)

but this is throwing some errors.

4

which way are you trying to go ?

cryosparc => relion, etc

or

relion, etc => cryosparc ?

5

I read it as “I have particles but no metadata”, which is a question which has cropped up a couple of times recently. If such is the case, whether going further is possible is dependent on the user knowing a fair bit about the provenance of their data…

6

I have a fair idea about my data like the pixel size, exposure dose, particle diameter etc. I just want to run the 2D classification job on cryosparc for which I require a metadata star file

7

nothing to do with relion just want to run 2D classification job.

8

So where did the data come from? CryoSPARC? cisTEM? EMAN2? Warp? SPHIRE? IMAGIC? Spider? :wink: