Merging two datasets having different pixels

Hello,
I wish to merge two datasets with different pixel sizes. So, there are mainly two ways of doing so-

  1. Merge the micrographs
  2. Merge the particles
    I need to merge the micrographs, so what can be done?
    Also, how to cross-check whether the pixel size has changed or not?

I know this topic has been discussed previously, but I am confused about the F-crop calculation.
Thank you.

Hello sky,
I happend to see this paper related with data merge:

hope it helps.
best luck
Ge

With a slight trade-off in your final resolution, it is possible to reduce the effective pixel size of both datasets and process them together. This helped me merge two datasets collected on the same grid and microscope settings but with different pixel sizes. I don’t know if it works for data collected on two different detectors, but in my case, I could merge and improve the final map resolution by this method and sharing the same here:

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Another way is to change one dataset (the smaller pixel size) using MotionCor3 which allows arbitrary -FtBin parameter (like 1.2717391304). Compared to the way @prafful explained the error is smaller because the spectral resolution of the micrographs is very fine, and Nyquist doesn’t need to be sacrificed as much, but it takes longer and involves different software. Doubt there is a difference in practice unless Nyquist is a serious concern.